HK-1-Luc cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

HK-1-Luc Order

Catalog#

YC-C095-Luc-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Luciferase Stable Cell Lines

cell name

HK-1-Luc

Morphology

Epithelial-like,?adherent

Passage ratio

1:2~1:4

Species

Human

Reporter gene

Luciferase

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Luciferase validation

Sample	Compound pore 1	Compound pore 2	Compound pore 3	Average	Expressed multiple
HK-1-Luc	1384935	1482437	638661	1168677.667	3161.434
HK-1	426	296	387	369.667	3161.434

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HK-1			STR Info (Cell bank) Cell Line:NPC/HK1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11.0	13.0
D13S317	11	11		11.0	11.0
D7S820	8	11		8.0	11.0
D16S539	9	11		9.0	11.0
VWA	18	19		18.0	19.0
TH01	7	9		7.0	9.0
AMEL	X	X
TPOX	8	11		8.0	11.0
CSF1PO	11	12		11.0	12.0

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the HK-1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HK-1-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
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Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
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High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
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